| Children |
| | 1. of Raecleuch Robert Johnstone, b. 1526, Lochwood Castle, Dumfriesshire, Scotland  d. 5 Oct 1592, Dumfriesshire, Scotland (Age 66 years) [Father: natural] [Mother: natural] |
| | 2. Lord of Johnstone Sir James "The Younger", b. Abt 1528, Johnstone Castle, Renfrewshire, Scotland d. Scotland [Father: natural] [Mother: natural] |
| | 3. Janet Johnstone, b. Abt 1530, Johnstone Castle, Renfrewshire, Scotland [Father: natural] [Mother: natural] |
| | 4. of Harthope Howcleuch and Raec John Johnstone, b. Abt 1540, Johnstone Castle, Renfrewshire, Scotland [Father: natural] [Mother: natural] |
| | 5. John of Over Cogrie Johnstone, b. Abt 1540, Johnstone Castle, Renfrewshire, Scotland d. 23 Sep 1603, Scotland (Age ~ 63 years) [Father: natural] [Mother: natural] |
| | 6. Dorothea Johnstone, b. Abt 1540, Johnstone Castle, Renfrewshire, Scotland [Father: natural] [Mother: natural] |
| | 7. Margaret Johnstone, b. Abt 1540, Johnstone Castle, Renfrewshire, Scotland [Father: natural] [Mother: natural] |
| | 8. of Hardgraif James Johnstone, b. Abt 1540 [Father: natural] [Mother: natural] |
| | 9. David Johnstone, b. Abt 1540 [Father: natural] [Mother: natural] |
| | 10. Elizabeth Johnstone, b. Abt 1558, Johnstone Castle, Renfrewshire, Scotland [Father: natural] [Mother: natural] |
|
Abt 1507 - 1567 (~ 60 years)
