Ancestry Research in the United Kingdom
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 | Lord of Johnstone John Johnstone (1507-1567) |
 | Elizabeth Jardine (1511-1544) |
| of Raecleuch Robert Johnstone (1526-1592)  |
| Janet Johnstone (1530- ) |
| John of Over Cogrie Johnstone (1540-1603) |
| Dorothea Johnstone (1540- ) |
| Margaret Johnstone (1540- ) |
| of Hardgraif James Johnstone (1540- ) |
| David Johnstone (1540- ) |
| Elizabeth Johnstone (1558- ) |
| Margaret Hamilton (1528- ) |
| of Johnstone and Dunskellie Sir John Johnstone (1544-1587) |
| Jean Johnstone (1550-1608) |
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