Ancestry Research in the United Kingdom
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 | Sir James Johnstone (1567-1608)
|
 | Sarah Maxwell (1576-1636) |
| Agnes Johnstone (1600- ) |
| Elizabeth Johnstone (1600- ) |
| James Johnstone, Lord of Lockwood (1602-1653)
|
| Lady Margaret Douglas (1605-1642) |
| James Johnstone, 1st Earl of Annandale (1625-1672)  |
| Col. William of Blacklaws Johnstone (1625-1656) |
| Mary Johnstone (1630-1680) |
| Margaret Johnstone (1630-1655) |
| Janet Johnstone (1630-1675) |
| Bethia Johnstone (1640- ) |
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